Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.

نویسندگان

  • G E Smith
  • G Ju
  • B L Ericson
  • J Moschera
  • H W Lahm
  • R Chizzonite
  • M D Summers
چکیده

A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.

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عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 82 24  شماره 

صفحات  -

تاریخ انتشار 1985